effect of leptin receptor q223r polymorphism on breast cancer risk

objective(s):leptin receptor (lepr) is a member of the class i cytokine receptor super-family that is known implicated in the initiation and progression of breast cancer. we have investigated the effect of q223r polymorphism on the breast cancer susceptibly in a sample of iranian subjects. materials and methods:we utilized a polymerase chain reaction (pcr) restriction fragment length polymorphism (rflp) method to investigate the association ofleprq223r polymorphism with breast cancer risk in a case control study consisting of 100 breast cancer cases and 100 controls without breast cancer. serum levels of leptin and soluble leptin receptor (sob-r) were measured by elisa method. results:the genotype (qq, qr, and rr) distributions were 25, 56, and 19 % in breast cancer cases and 54, 40, and 6% in controls, respectively. the frequency of 223 rr genotype was significantly elevated in breast cancer cases as compared to controls (χ2= 20.072, p<0.001). similar significance differences were also found in allele frequencies for q and r between two groups (χ2= 19.027, p< 0.001). additionally, there weresignificant association between q223r genotypes and breast cancer risk; homozygotes for rr genotype (or= 6.840; 95% confidence interval [ci] = 2.434-19.218), heterozygotes for qr (or=3.024; 95% ci = 1.620-5.644, p = 0.001), and qr+rr genotype (or= 3.522; 95% ci = 1.934-6.414, p < 0.001), respectively. conclusion:our results showed thattheleprq223r polymorphism is associated with increased breast cancer risk as well as tumor grade in a sample of iranian subjects.